Imaging Seminar: Simultaneous 3D imaging in Biology with Multifocus Microscopy
Event details
| Date | 12.10.2023 |
| Hour | 17:00 › 18:00 |
| Speaker | Prof. Sara Abrahamsson, University of California Santa Cruz (US) |
| Location | |
| Category | Conferences - Seminars |
| Event Language | English |
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Abstract. The conflict between the three-dimensional nature of the world and the two-dimensionality of the microscope image is a classical problem in biological imaging and in the field of Optics. Aberration-corrected multifocus microscopy (MFM) uniquely addresses this problem by truly simultaneously acquiring data from multiple planes of an object at the full resolution that the optical microscope can attain. Using diffractive Fourier optics, the image beam from a wide-field microscope is multiplexed and refocused and the 3D focal stack is captured on one or multiple high-speed cameras. MFM is compatible with super-resolution and localization microscopy as well as polarization microscopy and provides high-speed 3D imaging of living specimens without the need for image reconstruction.
Biography. Sara Abrahamsson received her PhD in 2012 from the University of California San Francisco in the laboratory of Mats Gustafsson and was a postdoc and Leon Levy fellow at the Rockefeller University in New York in the laboratory of Cori Bargmann. In 2017, she became Assistant Professor at the University of California Santa Cruz, where her group designs and develops 3D and super-resolution microscopy systems to visualize the dynamic processes of life with high spatial and temporal resolution. Applications of her group’s imaging systems range from functional neuronal imaging in small model organisms to visualizing transcription inside the cell nucleus.
The talk is followed by an aperitif.
Registration appreciated
More info here
Abstract. The conflict between the three-dimensional nature of the world and the two-dimensionality of the microscope image is a classical problem in biological imaging and in the field of Optics. Aberration-corrected multifocus microscopy (MFM) uniquely addresses this problem by truly simultaneously acquiring data from multiple planes of an object at the full resolution that the optical microscope can attain. Using diffractive Fourier optics, the image beam from a wide-field microscope is multiplexed and refocused and the 3D focal stack is captured on one or multiple high-speed cameras. MFM is compatible with super-resolution and localization microscopy as well as polarization microscopy and provides high-speed 3D imaging of living specimens without the need for image reconstruction.
Biography. Sara Abrahamsson received her PhD in 2012 from the University of California San Francisco in the laboratory of Mats Gustafsson and was a postdoc and Leon Levy fellow at the Rockefeller University in New York in the laboratory of Cori Bargmann. In 2017, she became Assistant Professor at the University of California Santa Cruz, where her group designs and develops 3D and super-resolution microscopy systems to visualize the dynamic processes of life with high spatial and temporal resolution. Applications of her group’s imaging systems range from functional neuronal imaging in small model organisms to visualizing transcription inside the cell nucleus.
The talk is followed by an aperitif.
Registration appreciated
More info here
Practical information
- General public
- Free
Organizer
- EPFL Center for Imaging