Spying on the inner workings of cells with super-resolution microscopy
Event details
| Date | 13.04.2015 |
| Hour | 16:15 › 17:15 |
| Speaker |
Prof. Suliana Manley, Lab. of experimental biophysics, EPFL Bio: From 06/2009 Tenure-track assistant professor, EPFL, Lausanne, Switzerland 2006-2009 Post-Doctoral fellow, National Institutes of Health, Bethesda, MD, USA 2004-2006 Post-Doctoral fellow, Massachusetts Institute of Technology, Cambridge, MA, USA 1999-2004 PhD (Physics) Awarded 06/2004, Harvard University, Cambridge, MA, USA 1993-1997 Bachelors (Cum Laude) Physics & Mathematics, Rice University, Houston, TX, USA |
| Location | |
| Category | Conferences - Seminars |
Our primary goal is to use experiments to inform quantitative models for the biophysics of protein assemblies, in the cellular environment. Toward this goal, we have developed super-resolution and single-molecule fluorescence imaging technologies. We have enabled automated 3D PALM acquisition, developed algorithms for rapid and quantitative analysis, and identified better dyes and buffers for robust multicolor imaging. With these technological breakthroughs, we have made several exciting biological findings. We showed that the bacterial division machinery is patchy across the cell cycle and quantified its architecture for hundreds of cells, constraining its potential role in generating force during cell division. We also revealed the symmetry of the core centriolar protein complex, a structure that is key to proper cell polarization and division. We are now poised to take these studies to the next level with large-scale, high throughput microscopy.
Practical information
- General public
- Free
Organizer
- Section de Physique
Contact
- Valérie Schaerer Businger