Genetically Programmed Synthesis of non-natural Polymers and Macrocyclic (Depsi-) peptides
Abstract
Recent advancements in whole genome synthesis of an artificial E. coli cell with a reprogrammed genetic code have liberated three codons for reassignment. These "blank" codons can be reassigned to encode various non-canonical amino- and/or alpha-hydroxy acids. The reassignment of multiple orthogonal tRNA/-synthetase pairs, including hydroxy acid selective variants, allows for the rapid, genetically programmed synthesis of diverse non-canonical polymers and (depsi-) peptide macrocycles. This provides a promising platform for the discovery of artificial polymers and macrocycles, demonstrating the potential for creating biochemically diverse molecules within a living cell.
Recent advancements in whole genome synthesis of an artificial E. coli cell with a reprogrammed genetic code have liberated three codons for reassignment. These "blank" codons can be reassigned to encode various non-canonical amino- and/or alpha-hydroxy acids. The reassignment of multiple orthogonal tRNA/-synthetase pairs, including hydroxy acid selective variants, allows for the rapid, genetically programmed synthesis of diverse non-canonical polymers and (depsi-) peptide macrocycles. This provides a promising platform for the discovery of artificial polymers and macrocycles, demonstrating the potential for creating biochemically diverse molecules within a living cell.
Practical information
- General public
- Free
Contact
- Marta Ruiz Cumi: [email protected]